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1. How can I contact CaptureSelect customer service?
2. Can we order by fax or e-mail
3. I want to change my password
4. I have not received an e-mail confirming my order is being processed
5. I have not received my order confirmation including prices and delivery
6. Shipment not arrived
7. Shipment not arrived in good order
8. Change your contact information
9. When is the invoice send?
10. What are the delivery timings?
11. Can I cancel an order?

Our CaptureSelect customer service team can be contacted by email at the following adress ligands@captureselect.com 

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Ordering by fax or e-mail is possible; however we prefer online ordering. Please follow this link for the online fax or email form.

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If you want to change your password please go to my account and fill in your new password.

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If you have not received an e-mail stating your order is being processed, please contact us.

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If you have not received your order confirmation within 3 working days, please reply to your order transmission e-mail to contact us and detail the problem.

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If a shipment has not arrived on or before the delivery date stated on the order confirmation please reply to the order confirmation e-mail detailing the problem.

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If a shipment has not arrived in good order please reply to the order confirmation e-mail detailing the information of your order and explain the problem.

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You can change your contact information by clicking on my account after log in.

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The invoice will be send by regular mail as soon as the order is shipped, and normally arrives around the same day as the package. For all other invoice questions please contact us.

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The order will be processed immediately and the delivery date can be found in the order confirmation. Normally delivery takes place within 10 working days.

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An order can be canceled but this has to be done before the product has been shipped. To cancel send a reply to your order confirmation e-mail (automatically detailing all ordering information). Please clearly state ‘Cancellation’ and explain your reasons for cancelling the order. We may calculate an administration fee for the cancellation. The order has not been cancelled until you have received a confirmation of the cancellation.

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1. What about BioProcess?
2. What is the advantage of CaptureSelect human Fc compared to Protein A?
3. What is the specificity of the different products from the antibody toolbox affinity matrices?
4. How to perform affinity chromatography with CaptureSelect antibody toolbox matrices?
5. How to neutralize the elution fractions of the antibody toolbox affinity matrices?

The antibody toolbox affinity matrices are intended for research applications. If your are interested in using the Human Fc affinity matrix and the Fab Kappa affinity matrix  for BioProcess applications please click here.

For use of the Fab Lambda and Multi species Fc affinity matrix in BioProcess application please contact us.

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CaptureSelect human Fc is specific for human IgG and binds all four human IgG subclasses (IgG _1-4) with high affinity, while Protein A does not bind IgG3.

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The CaptureSelect antibody toolbox consists of four different affinity matrices with different specificities.

CaptureSelect Human Fc: Is specific for human IgG and recognizes all four subclasses. Besides this application, the matrix is ideal for the purification of recombinant human Fc fusion proteins.

CaptureSelect Multi Species IgG: Is specific for IgG and recognizes IgG of at least 17 different species.

CaptureSelect Fab kappa: Is specific for human kappa light chains and binds human IgG, IgA, IgM, and IgE with kappa light chains. Also free kappa light chains are bound by this matrix.

CaptureSelect Fab lambda: Is specific for human lambda light chains and binds human IgG, IgA, IgM, and IgE with lambda light chains. Also free lambda light chains are bound by this matrix.

When the Fab lambda and Fab kappa affinity matrices are used in combination this will result in full human Ig purification.

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1) Before use, the affinity media must be equilibrated into a suitable binding buffer. A good general binding buffer is PBS, pH 7.2-7.4 (physiological pH & ionic strength). Ultimately, the most suitable binding conditions need to be determined empirically.

2) Sample can be applied on the column. The optimal binding condition for samples is around physiological pH. Samples with acidic pH should be avoided, since binding of the target molecule will not occur at these conditions. The amount of sample that can be loaded is depending on the concentration of the target molecule in your sample and the dynamic binding capacity of the matrix. The dynamic binding capacity for human IgG is 10 mg per ml matrix.

3) After sample application, the column should be washed with binding buffer until baseline has been re-established on the monitor. A typical wash is 5-10 column volumes. 4) Elution of the target molecule from the affinity media is achieved by an acidic elution buffer, for example 0.1 M Glycine pH 3.0. If the elution is not complete or the elution peak shows tailing, elution with 0.1 M Glycine pH 2.0 may solve this problem. For CaptureSelect Fab kappa affinity matrix 0.1 M Glycine pH 2.0 is recommended.

5) The affinity matrix should be re-equilibrated in binding buffer and is ready to use for a second affinity purification run. If the column will not be used immediately, the matrix should be stored in 20% ethanol at 4 °C (39 °F).

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After the acidic elution of the bound proteins from the antibody toolbox affinity matrices, the elution fractions can be neutralized with 1.0 M Tris pH 8.0 by adding 0.1 volume of  the elution fraction. This results in 100 mM Tris pH 8.0, final concentration. The 10 times concentrated neutralizing buffer can also already be added to the tubes in which the elution fractions will be collected, which shortens the time that the eluted protein is exhibited to low pH.

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