Antibody Toolbox

This range of research products removes the uncertainty involved in selecting the most appropriate purification media for your application. Standardized purification protocols minimize testing and optimization stages.

These specifically designed ligands are conveniently immobilized on high quality sepharose resins. The following formats can currently be purified: human IgG, human Fab fragments, IgG (several species), human IgA, IgM and human IgG4.

CaptureSelect® Human Fc

This affinity matrix is specifically designed to purify all human IgGs (including IgG3) and Fc fusion proteins from cell culture and transgenic expression systems, as well as from human serum and plasma. The matrix is based on a proprietary ligand that recognizes all four subclasses of human IgG through high affinity binding at the Fc region of the heavy chain. Unlike Protein A this product has a high specificity for human IgGs, providing the ability to separate human IgG populations (both monoclonal and polyclonal) from transgenic expression systems. In addition, in contrast to Protein A this product also can purify human IgG3 along with human IgG 1, 2 and 4, and it can be used to purify polyclonal human IVIG from plasma and serum.

CaptureSelect® Fab kappa

This affinity matrix is specifically designed to purify all human kappa Ig light chain (subclass I, II, III and IV) containing antibody formats and fragments, including kappa Fab, kappa Fab2’, and all subclasses of human kappa IgG, IgA, IgD, IgE, and IgM. The matrix is based on a proprietary ligand that recognizes human kappa light chains through high affinity binding at the light chain constant domain. Unlike protein L which binds the variable domain and ignores a significant fraction of the kappa light chain population, this product binds 100% of human kappa light chains. In contrast to protein L this product has a high specificity for human kappa light chains, providing the ability to separate human kappa light chain populations from transgenic expression systems. Furthermore, this product can also be used to elute under gentle elution conditions.

CaptureSelect® Fab lambda

This affinity matrix is specifically designed to purify all human lambda Ig light chain (subclass I, II, III and IV) containing antibody formats and fragments, including lambda Fab, lambda Fab2’, and all subclasses of human lambda IgG, IgA, IgD, IgE, and IgM. The matrix is based on a proprietary ligand that recognizes human lambda light chains through high affinity binding at the light chain constant domain. This product has a high specificity for human lambda light chains, providing the ability to separate human lambda light chain populations from transgenic expression systems. This product can also be used to elute under gentle elution conditions.

CaptureSelect® Multi Species Fc

This affinity matrix is specifically designed to purify IgGs from multiple species including mouse, cow, rabbit, rat, goat, horse, human and sheep. For a complete list of species refer to the product sheet. The matrix is based on a proprietary ligand that recognizes IgGs from multiple species through high affinity binding at a highly conserved epitope on the Fc region of the heavy chain. Unlike Protein, G, which, under standard elution conditions, is known to present challenges that result in poor yield, dissociated homodimers, and denatured antibodies, this product can be used with gentle elution conditions, retaining the structural and functional integrity of the eluate.

CaptureSelect® Human IgA

The human IgA affinity matrix provides superior performance over other IgA purification resins in terms of specificity, purity, and dynamic binding capacity. The ligand is coupled to NHS-activated Sepharose 4 Fast Flow™ (GE Healthcare). This matrix can be used for the purification and isolation of human IgA from complex sources like plasma, serum, and cell culture supernatants.

CaptureSelect® Human IgM

The CaptureSelect IgM affinity matrix contains a ligand recognizing a unique domain on the Fc part of IgM that enables purification of human and mouse IgMs without cross reactivity with human and mouse IgG and IgA. This makes the CaptureSelect IgM affinity matrix a superior resin in comparison with other IgM matrices in terms of specificity and product purity. The ligand is coupled to NHS-activated Sepharose 4 Fast Flow™ (GE Healthcare). This matrix can be used for the purification and isolation of IgM, without cross-binding to other immunoglobulins, from complex sources like plasma, serum, and cell culture supernatants.

CaptureSelect® Human IgG4

This CaptureSelect affinity resin is designed to uniquely recognize human IgG4. The resin contains a ligand directed towards a unique domain on the Fc part of IgG4. This enables purification of human IgG4 without cross reactivity with human IgG subclasses 1, 2 or 3. CaptureSelect IgG4 affinity matrix has an unparalleled specificity allowing one-step purification. The ligand, coupled to NHS-activated Sepharose 4 Fast Flow™ (GE Healthcare), also shows no cross-binding with other immunoglobulins, making it suitable for IgG4 purification and isolation from complex sources like plasma, serum, and cell culture supernatants. To purchase any of these affinity matrices visit our Web Shop at www.captureselect.com.