VIIISelect leakage assay

VIIISelect affinity medium (GE Healthcare) is designed for the purification of recombinant B domain depleted Factor VIII. The CaptureSelect VIIISelect affinity ligand is based on a single chain antibody fragment that was developed by screening for unique selectivity for human Factor VIII. The ligand, a 13 kD recombinant protein, is coupled with multipoint attachment through stable amide bonds to give high chemical stability and low leakage. Even when covalently attached, small amounts of the VIIISelect affinity ligand can leach off of the chromatography support and co-elute with human Factor VIII. This ELISA provides sensitivity to detect VIIISelect affinity ligand contamination to less than 1 ng/ml. This assay is designed to minimize interference and to provide accurate quantitation in the presence of human Factor VIII. This kit can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.

Please go to our online ordering module order to learn more about this product

« back to products